Lucie Kretzler studied medicine at the Charles University in Prague. During her residency at the HELIOS Clinic in Pirna from 2012 to 2017, she completed a doctoral programme at the Technical University of Dresden, graduating magna cum laude. The topic of her dissertation was "Analysis of baseline characteristics and clinical outcomes of patients undergoing percutaneous left atrial appendage occlusion at Pirna Hospital". From 2017 to 2020, Lucie Kretzler worked as a specialist in internal medicine and cardiology at the Charité Campus Virchow-Klinikum. Since 2020, she has been head of the Cardiovascular Assessment Module at the Clinical Study Centre (CSC) of the Berlin Institute of Health (BIH).